Oxford Nanopore Sequencing
The emergence of sequencing technologies like Nanopore (ONT) has transformed microbial detection, prompting our commitment to advancing clinical and agricultural diagnostics through innovative fungal profiling assays. Fungal infections pose escalating threats in medicine and agriculture, given their integral role in disease progression and the complexity of microbial communities. Existing microbiota analysis tools, primarily designed for bacterial detection (e.g., targeting the 16S rRNA gene), often prove inadequate for fungal investigations reliant on marker genes like the full-length ribosomal operon, encompassing ITS regions.
Our objective is to facilitate rapid, precise, and economical diagnostic assays tailored for high-throughput sequencing platforms. Employing pioneering primers for specimen preparation in Nanopore sequencing and leveraging bioinformatics tools, we strive to enhance the sensitivity and specificity of our assays.


Our current research concentrates on grapevine mycobiota, notably Botrytis cinerea, Alternaria alternate, and Saccharoses (LC750323.1, LC749799.1, LC749799.1, LC743580.1), utilizing the full-length ribosomal operon spanning 5800 bp to glean critical insights into fungal infection diagnosis.Continuing our investigations, in collaboration with New England Biolabs (NEB), we endeavour to diversify our portfolio to encompass pathogens affecting plants, humans, and animals.