May 3, 2023

Synthetic Primers for Disease Diagnostics

We are continuously exploring and developing new technologies to advance the field of high-throughput diagnostics. Our team of researchers is dedicated to creating custom oligonucleotides that meet your precise specifications, while also pushing the boundaries of what is possible on the market.

We are using the latest technologies and techniques to produce Oligos that are both highly accurate and efficient, with fast turnaround times and rigorous quality control processes. Our advanced manufacturing processes allow us to produce Oligos at scale, while still ensuring that each Oligo is made to the highest standards of quality.

Our Primer Design and Optimisation Process Includes the following steps:

  1. Selection of the target sequence and designing specific primer sets: Our team of experts use their knowledge and expertise to select the most appropriate target sequence, and design primer sets specific to the target sequence.
  2. Bioinformatics analysis: Bioinformatics is done to guarantee that the target sequence will be amplified effectively and precisely by the primers.
  3. Synthetic primer synthesis: We use cutting-edge chemistry to synthesize the primers after they have been created and validated in In-silico. Modern Oligo synthesis techniques guarantee that the primers are high quality and efficient.
  4. In-vitro analysis: Laboratory experimental validation of designed primers through PCR amplification testing is the final step in our primer design and optimization process. We use different downstream applications, such as PCR, RT-qPCR, NGS, etc. to validate the efficiency and specificity of our primer sets. The testing also involves optimizing PCR conditions, such as reagent concentrations and annealing temperatures. The testing ensures amplification succeeds and avoids primer dimers and PCR chimera formation.

The importance of using high-quality primers for downstream applications is gaining traction. Therefore, we chose multi-step experimental processes so that our synthetic primers are of the highest quality and validated for various new-generation molecular biology assays.

In this article:
Our team pioneers high-quality oligonucleotides, employing advanced techniques for precise customization and rigorous quality control to meet market demands.
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